Abstract
The use of 5′-triphosphate of 1-(2-deoxy-β-D-ribofuranosyl)imidazole-4-carboxamide (dYTP) in DNA amplification reaction in place of dATP or dGTP yielded mutations frequencies of 3–4×10−2 per base per amplification. A reasonable proportion of transversions (11–15%) was observed in the absence of deletions and insertions.