Abstract
An optimized automated PNA synthesis protocol is reported. Under optimal conditions the product yield of a test 17-mer PNA is approximately 90 %. The average coupling yield is 99.4 %. The synthesis strategy is Boc/Z. The protocol is developed in a 5 pmole scale but is easily scaled up to 10–50 μmole scale syntheses on the automated synthesizer (ABI 433A). DNA capture experiments by PNA was used to develop a method for PNA-mediated purification of genomic Chlamydia DNA from urine. This purification removed efficiently substances that impeded DNA amplification.
∗PNA diagnostics owns the world-wide exclusive rights to PNA in diagnostics and monitoring, in fields such as human clinical/forensic medicine, veterinary diagnostics, food/agriculture, and environmental monitoring.
Notes
∗PNA diagnostics owns the world-wide exclusive rights to PNA in diagnostics and monitoring, in fields such as human clinical/forensic medicine, veterinary diagnostics, food/agriculture, and environmental monitoring.