LncRNA LINC00958 Activates mTORC1/P70S6K Signalling Pathway to Promote Epithelial-Mesenchymal Transition Process in the Hepatocellular Carcinoma

Abstract

The study aimed to investigate the influence of LINC00958 on the EMT process of hepatocellular carcinoma (HCC). In our study, The LINC00958 was up-regulated in HCC tissues and cell lines. LINC00958 silencing inhibited cell proliferation, migration, and EMT process of HCC. The analysis of TCGA and StarBase showed that NUDT19 was a direct target of LINC00958 and was positively regulated by LINC00958. Besides, NUDT19 activated mTORC1/P70S6K signalling pathway. Both NUDT19 overexpression and mTORC1 activator MYH1485 reversed the inhibitory effect of LINC00958 silencing on proliferation, migration, and EMT process of HCC. In conclusion, LINC00958 silencing inhibited the proliferation, migration, and EMT process of HCC via inhibiting NUDT19 mediated mTORC1/P70S6K signalling pathway.

Keywords:

• Hepatocellular carcinoma
• LINC00958
• NUDT19
• mTORC1
• EMT

Ethics approval and consent to participate

The protocol of this research has been approved by the Ethics Committee of QingDao No. 6 People’s Hospital. All patients have signed written informed consent.

Author contributions

Chuangxia Lan and Yanming Wang designed the study and performed the research; Xiaofei Su, Jing Lu and Saisai Ma analyzed data and wrote the paper. All authors have read and approved the manuscript.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Data availability statement

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.