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Journal of Biomolecular Structure and Dynamics Volume 25, 2008 - Issue 6
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Original Articles

Influence of Ionic Strength on Hoechst 33258 Binding with DNA

Poghos O. Vardevanyan Department of Biophysics, Yerevan State University, Yerevan, 375025, Armenia
,
Ara P. Antonyan Department of Biophysics, Yerevan State University, Yerevan, 375025, Armenia
,
Marine A. Parsadanyan Department of Biophysics, Yerevan State University, Yerevan, 375025, Armenia
,
Kristine V. Pirumyan Department of Biophysics, Yerevan State University, Yerevan, 375025, Armenia
,
Andranik M. Muradyan Physics Department, Yerevan State University of Architecture and Construction, Yerevan, 375009, Armenia
&
Armen T. Karapetian Physics Department, Yerevan State University of Architecture and Construction, Yerevan, 375009, Armenia
Pages 641-646 | Received 24 Oct 2007, Published online: 15 May 2012
 
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Abstract

The binding of Hoechst 33258 with DNA at various ionic strengths of solution and different ligand concentrations has been investigated. Existence of more than one type of interactions of Hoechst 33258 with DNA has been revealed, which were very sensitive to the ionic strength. Hoechst 33258 doesn't show specificity to AT sequences of DNA at low ionic strength. High affinity binding mode becomes obvious at high ionic strength. The values of binding constants and binding site sizes for revealed strong and weak interactions have been determined.

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Related Research Data

Targeting the minor groove of DNA
Source: Elsevier BV
Molecular design of DNA-directed ligands with specific interactions: solution NMR studies of the interaction of a m-hydroxy analogue of Hoechst 33258 with d(CGCGAATTCGCG)2.
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