A comparison on the biochemical activities of Fluorescein disodium, Rose Bengal and Rhodamine 101 in the light of DNA binding, antimicrobial and cytotoxic study

Abstract

Biochemical activities of Fluorescein, Rose Bengal and Rhodamine 101 were studied by DNA binding, antibacterial and cytotoxic studies. DNA binding studies were done using spectroscopic, thermodynamic and molecular modeling techniques. Antibacterial activities were investigated against a gram-negative bacteria Escherichia coli and a gram-positive bacteria Staphylococcus aureus. Cytotoxic activities were studied against Wi-38 cell line. We observed these dyes bound to minor groove of DNA and structural diversity of dyes affect the phenomenon. No significant antibacterial and cytotoxic activities of these dyes were found in our observations.

GRAPHICAL ABSTRACT

Communicated by Ramaswamy H. Sarma

Keywords:

• Xanthenegroup of dyes
• DNA-binding
• thermodynamic properties
• antibacterial
• cytotoxicity

Disclosure statement

No potential conflict of interest was reported by the authors.

Acknowledgements

The authors would like to thank Prof. Subrata Mukhopadhyay, Department of Chemistry, Jadavpur University. The authors also thank Mr. Asif Hasan, department education (post graduating), ABS academy, Durgapur for his valuable help.

Additional information

Funding

Mukti Mohammad and Md. Maidul Islam wish to thank Department of Science and Technology and Biotechnology (DSTB), Govt. of West Bengal for their financial support (Project No:756 (Sanc.)/ST/P/S&T/9G-34/2013). Mukti Mohammad thanks Govt. of India for partial financial support (F.No. 01-01/2019-Sch). Authors also acknowledge the partial financial help of Aliah University.