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Research Article

Identification of a Transcriptionally Inactive p53 Mutant by Functional Analysis of separated Alleles in Yeasts (Fasay) in a Child Osteosarcoma Tumor: A Case Report

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Pages 321-328 | Published online: 09 Jul 2009
 

Abstract

Tumor suppressor gene p53is one of the most specific genetic alterations occurring in osteosarcoma pathogenesis. It is thought to be an early and key step in the tumorigenesis of osteosarcoma. However, whether the p53 status is a marker predictive of response to therapy and a marker of prognostic value remains controversial. The choice of p53 status detection method certainly account for discrepancies. The authors used a simple functional assay (functional analysis of separated allelles in yeast) on the tumor sample of an 8-year-old girl presenting with an osteosarcoma of the tibia. While making it possible to exclude the presence of a germline mutation, FASAY indicated the presence of a somatic p53mutation lacking transcriptional activity on p21 and bax target genes. FASAY also strongly suggested a loss of heterozygosity p53, which was confirmed by cytogenetic analysis. Sequencing of cDNA extracted from yeast colonies containing mutated p53identified a 213 stop mutation in exon 6. Despite these p53alterations, the child is still in complete remission after a follow-up of 48 months.

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