PANEL REACTIVE ANTIBODY IN THALASSEMIC SERUM INHIBITS PROLIFERATION AND DIFFERENTIATION OF CORD BLOOD CD34⁺ CELLS IN VITRO

Jian-Pei Fang Department of Pediatrics, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

, MD,

Lu-Hong Xu Department of Pediatrics, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

, PhD,

Xing-Ge Yang Department of Pediatrics, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

, MD,

Yan-Feng Wu Department of Pediatrics, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

, BS,

Wen-Jun Weng Department of Pediatrics, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

, MD &

Hong-Gui Xu Department of Pediatrics, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

, MD

Abstract

Thalassemic children are at a high risk of graft rejection in cord blood transplantation. To investigate this possible mechanism, the authors evaluated the effect of panel reactive antibody on the growth of CD34⁺ cells in vitro. On semisolid medium, CD34⁺ cells derived from cord blood were incubated with thalassemic serum, and colony-forming units were counted after 10 days of culture. After incubation with serum-specific panel reactive antibody, profound decreases were found in the numbers of CFU-GM, CFU-GEMM and BFU-E compared with controls. The results indicated that serum-specific panel reactive antibody might have an apparent inhibition effect on proliferation and differentiation of cord blood CD34⁺ cells.

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PANEL REACTIVE ANTIBODY IN THALASSEMIC SERUM INHIBITS PROLIFERATION AND DIFFERENTIATION OF CORD BLOOD CD34⁺ CELLS IN VITRO

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PANEL REACTIVE ANTIBODY IN THALASSEMIC SERUM INHIBITS PROLIFERATION AND DIFFERENTIATION OF CORD BLOOD CD34+ CELLS IN VITRO

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PANEL REACTIVE ANTIBODY IN THALASSEMIC SERUM INHIBITS PROLIFERATION AND DIFFERENTIATION OF CORD BLOOD CD34⁺ CELLS IN VITRO