https://orcid.org/0000-0001-6670-1959
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ABSTRACT
Advanced glycation end products (AGEs) are toxic with a high capacity to cause damages to health, mainly associated with diabetes complications. In this study, soybean isoflavone extracts were subjected to different biotransformation processes: Lactobacilli fermentation, Enzymatic and Enzymatic followed by fermentation, in order to improve their antioxidant capacity and bioactive isoflavone content. The effects of the biotransformation processes on total phenolic content, antioxidant activities, and isoflavone profile were evaluated. Analyses of antiglycation activity of the isoflavones extracts were evaluated by glycation models in vitro. The results showed that all bioprocesses increased the aglycone content, as well as the antioxidant capacity by about 2.5 times using ORAC and 1.6 times using FRAP assays. The results were superior for enzymatic biotransformation followed by fermentation. All processes showed about 24,33% to 57,33% of anti-glycationt capacity, indicating that biotransformation improved the bioactivity of isoflavones extracts for glycation inhibitory activity, as well as the associated e antioxidant capacity.
Abbreviations
| AGEs | = | Advanced glycation end products |
| ARG | = | Arginine |
| BSA | = | Bovine serum albumin |
| CFU | = | Colony formation unit |
| DPPH | = | 2,2-diphenyl-1-picrylhydrazyl |
| FRAP | = | ferric reducing antioxidant power |
| FRU | = | Fructose |
| GAE | = | gallic acid equivalents |
| GO | = | Glyoxal |
| HPLC | = | high performance liquid chromatography |
| MGO | = | Methylglyoxal |
| ORAC | = | Oxygen radical absorbance capacity |
| TE | = | Trolox equivalents |
| Trolox | = | 6-Hydroxy-2,5,7,8-tetramethylchromane-2- carboxylic acid |
| UV | = | Ultraviolet |
| v/v | = | volume/volume |
Supplementary material
Supplemental data for this article can be accessed on the publisher’s website.