Enhancement of natural killer activity and IFN-γ production in an IL-12-dependent manner by a Brassica rapa L.

Abstract

Certain food components possess immunomodulatory effects. The aim of this study was to elucidate the mechanism of the immunostimulatory activity of Brassica rapa L. We demonstrated an enhancement of natural killer (NK) activity and interferon (IFN)-γ production in mice that were orally administered an insoluble fraction of B. rapa L. The insoluble fraction of B. rapa L. significantly induced IFN-γ production in mouse spleen cells in an interleukin (IL)-12-dependent manner, and NK1.1⁺ cells were the main cells responsible for producing IFN-γ. Additionally, the results suggested that the active compounds in the insoluble fraction were recognized by Toll-like receptor (TLR) 2, TLR4, and C-type lectin receptors on dendritic cells, and they activated signaling cascades such as MAPK, NF-κB, and Syk. These findings suggest that B. rapa L. is a potentially promising immuno-improving material, and it might be useful for preventing immunological disorders such as infections and cancers by activating innate immunity.

Active compounds in the insoluble fraction of Brassica rapa L. induces IFN-γproduction in a IL-12-dependent manner and enhancement of NK activity.

Keywords:

• Brassica rapa L.
• dendritic cells
• immunomodulation
• interferon-γ
• natural killer cell activity

Abbreviations:

• APCs, antigen-presenting cells
• BMDCs, bone marrow-derived dendritic cells
• BW, body weight
• CFSE, carboxyfluorescein diacetate succinimidyl ester
• CLRs, C-type lectin receptors
• Con A, concanavalin A
• Cyto D, cytochalasin D
• DCs, dendritic cells
• EDTA, ethylenediamine-N,N,N′,N′,-tetraacetic acid
• ELISA, enzyme-linked immunosorbent assay
• ERK, extracellular signal-related kinase
• FBS, fetal bovine serum
• FITC, fluorescein isothiocyanate
• GM-CSF, granulocyte-macrophage colony-stimulating factor
• IFN, interferon
• IL, interleukin
• i.p., intraperitoneally
• JNK, jun kinase
• LPS, lipopolysaccharide
• MAPK, mitogen-activated protein kinase
• mAbs, monoclonal antibodies
• MHC, major histocompatibility complex
• mLNs, mesenteric lymph nodes
• NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells
• NK, natural killer
• PB, polymyxin B
• PBS, phosphate-buffered saline
• PE, phycoerythrin
• PGN, peptidoglycan
• PI, propidium iodide
• PPs, Peyer’s patches
• RPMI, Roswell Park Memorial Institute
• SD, standard deviation
• Syk, spleen tyrosine kinase
• TCR, T cell receptor
• TLRs, Toll-like receptors
• TNF, tumor necrosis factor
• WT, wild-type

Acknowledgements

We thank Yoji Sugiyama (Marutaka Co., Ltd) for providing us with B. rapa L. We are grateful to Y. Suzuki for her skillful support of our experiments. We thank Scott Lloyd, PhD, from Edanz Group (www.edanzediting.com/ac), for editing a draft of this manuscript.