Fabrication of polyhydroxybutyrate (PHB)/γ-Fe₂O₃ nanocomposite film and its properties study

Abstract

Magnetic separation has numerous advantages in isolating cancerous and normal cells used in the diagnosis and treatment sectors. Here, we produced magnetic nanocomposite films made of polyhydroxybutyrate (PHB)/magnetite nanoparticles (γ-Fe₂O₃), and the properties of the films by SEM, TEM, FTIR, DMTA, contact angle, and cellular analyses were investigated. The microscopic images showed uniform distribution of γ-Fe₂O₃ magnetic nanoparticles in polymeric matrix. The chemical bounds between magnetic nanoparticles and polymeric matrix demonstrated using the FTIR spectrophotometer. The DMTA and contact angle results indicated an increase in the glass transition temperature and hydrophilic properties of nanocomposites is achieved by increasing the magnetic nanoparticles amount in polymer matrix. The cellular results were showed that adhesion of cancer cells compared to normal cells was significantly enhanced by the induction of a magnetic field. These nanocomposite films can be used as a substrate for cellular adhesion and separation processes.

Keywords:

• Magnetic nanocomposite
• polyhydroxybuthyrate (PHB)
• γ-Fe₂O₃ nanoparticles
• physical and mechanical analyses
• cell separation