The effects of soybean isoflavones and 17β-estradiol in uterus and mammary glands of diabetic rat models

Abstract

The objective of this study was to evaluate the action of soy isoflavones and 17 beta estradiol on the extracellular matrix in the uterus and mammary gland of diabetic rats. Sixty adult female rats underwent ovariectomy, then randomized into seven groups of ten animals each: Non-diabetic: GI Sham control animals ovariectomized; and GII control ovariectomized that received propylene glycol vehicle. Diabetic: GIII Sham control diabetic animals ovariectomized; GIV ovariectomized diabetic animals receiving propylene glycol vehicle; GV diabetic ovariectomized animals treated with soy isoflavones (150 mg/kg by gavage); GVI ovariectomized diabetic rats treated with estrogen (17b-estradiol, 10 mg/kg, subcutaneously); GVII diabetic ovariectomized animals treated with soy isoflavones (150 mg/kg by gavage), and with estrogen (17b-estradiol, 10 mg/kg combination therapy). Treatments occurred during 30 consecutive days. After animals euthanasia, a portion of the uterus was immersed in liquid nitrogen for molecular biology analysis, the other portion of uterus and mammary glands were removed and processed for paraffin embedding. Soy isoflavones (GV) and 17b estradiol improved the production of compounds of extracellular matrix, such as small leucine-rich proteoglycans (SLRPs). The combination of both therapies had an additive effect in SLRPs expression. Soy isoflavones contribute to the uterine integrity of SLRPs of diabetic rats.

Chinese abstract

本研究的目的是评估大豆异黄酮和17β-雌二醇对糖尿病大鼠子宫和乳腺中细胞外基质的作用。对60只成年雌性大鼠行卵巢切除, 后随机分为7组, 每组10只。非糖尿病大鼠：GⅠ空白对照组切除卵巢；GⅡ对照组切除卵巢并接受丙二醇载体。糖尿病大鼠：GⅢ空白对照组糖尿病切除卵巢；GⅣ组糖尿病切除卵巢并接受丙二醇载体；GⅤ组糖尿病切除卵巢并给予大豆异黄酮治疗（150 mg/kg灌胃）；GⅥ组糖尿病切除卵巢并给予雌激素治疗（17β-雌二醇, 10 mg/kg, 皮下）；GⅦ组糖尿病切除卵巢并给予大豆异黄酮（150 mg/kg灌胃）加雌激素（17β-雌二醇, 10 mg/kg, 皮下）治疗。持续给药30天后, 处死大鼠, 将一部分子宫浸入液氮冷冻用于分子生物学检测, 余下的子宫和乳腺石蜡包埋。大豆异黄酮（GV）和17β雌二醇可改善细胞外基质化合物的产生, 如小富含亮氨酸的蛋白聚糖（SLRPs）。两种方法联合使用对SLRPs的表达有累加效应。大豆异黄酮有助于糖尿病大鼠SLRPs子宫完整性。

Keywords:

• Hyaluronic acid
• isoflavones
• 17β-estradiol
• diabetics type 1
• small leucine-rich proteoglycans (SLRPs)

Disclosure statement

Authors declare that there is no conflict of interests regarding the publication of this article.

Additional information

Funding

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo – (FAPESP).