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Abstract
We investigated the influence of extracts from the mackerel (BM-1) on the production of IL-2 and IFNγ from lymphocytes. Intracellular IL-2 or IFNγ of lymphocytes activated with anti-CD3 monoclonal antibody (MAb) and PMA, or anti-CD3 MAb and PHA were analyzed by a flow-cytometer. The number of IL-2 or IFNγ positive-lymphocytes (CD4 positive or CD8 positive) was significantly decreased when the lymphocytes were treated with BM-1. Moreover, the production of IL-2 or IFNγ from lymphocytes were determined by ELISA systems. BM-1 inhibited the production of IL-2 and IFN γ from lymphocytes in a dose-dependent manner. Furthermore, we observed the effects of BM-1 on the calcium mobilization of lymphocytes activated with anti-CD3 MAb. BM-1 did not effect the initial, rapid phase (EGTA-insensitive component) of the TCR-stimulated increase in [Ca2+]i. BM-1 inhibited the influx of extracellular Ca2+. These results suggest that BM-1 inhibits the production of IL-2 and IFNγ from lymphocytes via the suppression of calcium influx through plasma membrane channels.