Rapid detection of rifampicin in fish using immunochromatographic strips

ABSTRACT

In this study we developed a rapid and simple assay to detect RFP in fish using an immunochromatographic assay. We produced a sensitive and selective monoclonal antibody against RFP with a half-maximal inhibitory concentration (IC₅₀) of 10.7 ng/mL. Fish samples were extracted with ethyl acetate, then centrifuged at 8000 rpm for 10 min, and concentrated by drying over nitrogen. We found under optimal conditions, that the RFP cut-off limits for the test strip was 10 µg/mL in both 0.01 M phosphate buffered saline and fish samples, and each test could be completed within 5 min. Therefore, immunochromatographic test can be used as a reliable, rapid, and sensitive method for the detection of RFP in fish.

KEYWORDS:

• Immunochromatographic test
• monoclonal antibody
• rifampicin
• strip test

Acknowledgements

This work is financially supported National Key R & D Program (2016YFD0501208, 2016YFF0202300) and grants from Natural Science Foundation of Jiangsu Province and MOF (BE2016307, BK20150145, BX20151038, BK20140003, BE2014672, CMB21S1614, 201513006).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work is financially supported National Key R & D Program [2018YFC1602901].