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International Journal of Radiation Biology Volume 63, 1993 - Issue 2
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Original Article

Does the Enzymatic Conversion of DNA Single-strand Damage into Double-strand Breaks Contribute to Biological Inactivation of γ-irradiated Plasmid DNA

Y. Ventur Max-Planck-Institut für Strahlenchemie, Stiftstraße 34–36, D-4330, Mülheim an der Ruhr, Germany
&
D. Schulte-Frohlinde Max-Planck-Institut für Strahlenchemie, Stiftstraße 34–36, D-4330, Mülheim an der Ruhr, Germany
Pages 167-171 | Received 27 Apr 1992, Accepted 08 Sep 1992, Published online: 03 Jul 2009
 
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Abstract

Incubation of γ-irradiated plasmid DNA (pBR322) in a protein extract of E. coli CMK wild-type cells leads to the formation of double-strand breaks which contribute to biological inactivation by ⩾ 80%.

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