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Original Paper

Liquid chromatographic determination of thiamin in dairy products

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Pages 345-349 | Published online: 06 Jul 2009
 

Abstract

Concentrations of thiamin were determined in pasteurized and UHT-sterilized milks, yoghurt and kefir samples by high-performance liquid chromatography using a reversed-phase C-18 column connected to fluorescence detector. The linearity, recovery and reproducibility of the method were satisfactory. The thiamin content ranged from 0.305±0.02 to 0.252±0.03 mg/l in market milks. Yoghurt samples had higher concentration of thiamin (0.355±0.04 and 0.404±0.02 mg/l) than values found in market milks. The least thiamin content was found in kefir (0.175±0.03 mg/l) among the samples analyzed.

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