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Abstract
Chemical composition, antimicrobial activity and antioxidative proprieties were assessed in air-dried shoots of Haplophyllum tuberculatum. Samples were subjected either to organic fractionated extraction (hexane, chloroform and methanol) or hydrodistillation for essential oil extraction. We found that methanol extract (ME) was the most enriched in polyphenols and that flavonoïds were more concentrated in chloroform one (CE). Antimicrobial tests showed that organic extracts were more effective against Gram negative bacteria than Gram positive ones. The best antibacterial activity was exhibited by ME which was also active against Candida albicans. Gas chromatography/mass spectrometry analysis of extracted essential oil from air-dried shoots revealed 18 indentified components representing 93.46 % of total oil. The major components were hexadecanoic acid (40.21 %) and oleic acid (26.75 %). Antioxidative assays showed that H. tuberculatum essential oil exhibited a weak ferric reducing power and moderate free radical scavenging activity. Obtained data argued that H. tuberculatum shoots are sources of natural products of promising antimicrobial and antioxidative capacities.