Hericium VN, an undescribed compound isolated from Hericium erinaceus and its cytotoxic activity on human brain astrocytoma

Abstract

Hericium erinaceus is a species of mushroom with high nutritional value that is used mainly as food in tropical countries. Phytochemical study of H. erinaceus led to the isolation of an undescribed compound, named as hericium VN (1), together with nine known compounds, 1-(2-formyl-1-pyrrolyl)butanoic acid (2), herierin III (3), 5′-(methylthio)adenosine (4), adenosine (5), nicotinic acid (6), (22E,24R)-5α,8α-epidioxyergosta-6,9(11),22-trien-3β-ol (7), 5α,8α-peroxycerevisterol (8), (22E,24R)-5α,8α-epidioxy-egosta-6,22-diene 3-O-β-D-glucopyranoside (9), and cerevisterol (10) based on extensive analyses of HR-ESI-MS, 1D and 2D NMR spectra. The absolute configuration of compound 1 was determined by experimental combined with calculated electronic circular dichroism spectra. Compound 7 exhibited cytotoxic effects against brain tumor cell line CCF-STTG1 with the IC₅₀ value of 15.50 µM, compared to that of the positive control compound, doxorubicin, which showed IC₅₀ value of 15.84 µM.

Keywords:

• Hericium erinaceus
• hericium VN
• hericiaceae
• steroid
• cytotoxic activity

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was funded by Vietnam Academy of Science and Technology (VAST) under grant number ĐL0000.02/22-23.