Target gene identification and functional characterization of miR-1704 in chicken

Abstract

MiRNAs are small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. SNPs in miRNA genes may lead to phenotypic variation by altering miRNA expression and their targets. In this study, miR-1704 expression profiles in nine tissues at 1 d, 6 weeks and 16 weeks old Gushi chickens were detected. MiR-1704 was widely expressed in the detection of tissues. The expression in 1 d and 6 weeks old was low abundance, while its expression at 16 weeks was very high. An rs14668705 (C > G) SNP was detected within the pre-miR-1704 in an F₂ resource population of Gushi chicken crossed with Anka broiler. Bioinformatic analysis indicated that the C > G mutation could introduce a base-pair mismatch and cause the change of free energy. Experiments further revealed that the rs14668705 in precursor miR-1704 could significantly affect mature miR-1704 biogenesis and was significantly associated with body weight at the age of 0, 6, 8, 10, and 12 weeks, shank circumference at 4, 8, and 12 weeks, carcass weight, and semi-evisceration weight (p < 0.05). Insulin receptor 2 (IRS2) gene, one of the potential targets of miR-1704 was identified and further confirmed. These data suggested that miR-1704 targeted IRS2 and have an effect on body weight in chicken.

Keywords:

• miRNA
• miR-1704
• IRS2
• body weight
• polymorphism

Acknowledgments

Y. C. Wang and S. H. Wang contributed equally to this work.

Disclosure statement

The authors declare that they have no conflict of interest.

Additional information

Funding

This work was supported by the Program for Innovation Research Team of Ministry of Education [IRT16R23], Research on the Foundation and Frontier Technology of Henan Science and Technology Department [162300410162], and the Earmarked Fund for Modern Agri-industry Technology Research System [CARS-40-K04].