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ABSTRACT
Effect of pretreatment methods including ultrasonication (US), microwave (MW), and ultrafine milling (UM) on hydrolysis efficiency and antioxidative activity of peptides produced from bighead carp were investigated. The UM peptides showed higher hydrolysis efficiency (higher degree of hydrolysis, nitrogen recovery, and oligopeptide yield; narrower molecular-weight distribution), and higher 1,1-dipheny1-2-pycrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfomic acid) diammonium salt (ABTS) radical scavenging activities, and metal chelating activities than US and MW peptides. The hydrolysis efficiency and antioxidative activity of UM peptides could remain relatively high even when the substrate concentration increased up to 50%. In conclusion, ultrafine milling may be a suitable pretreatment method in the production of peptides.
Funding
This research was financially supported by the earmarked fund for China Agriculture Research System (CARS-46), National Natural Science Foundation of China (NSF31171709), and the Fundamental Research Funds for the Central Universities (No. JUSRP111A34).