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ABSTRACT
In the present study, antioxidative potential of squid protein hydrolysates (SPH) prepared using endogenous pepsin and trypsin prepared from the stomach and pyloric ceca of seer fish respectively was evaluated, in comparison with commercial enzymes. Degree of hydrolysis (DH) of the pepsin SPH ranged between 8–11% and trypsin SPH between 12–14% after 150 min of hydrolysis. SPH prepared with endogenous enzymes had significantly higher DH than commercial enzymes. Pepsinogen SPH expressed more DH than pepsin SPH. Trypsin SPH exhibited good metal chelating ability (98%), which was equivalent to EDTA at 130 ppm. Pepsin SPH showed higher super oxide anion scavenging activity (75%). Trypsin and pepsin SPH also had high DPPH radical scavenging (85%), ABTS radical scavenging (93%) and ferric reducing (Abs700 = 1.2) abilities. Thus, metal chelating and superoxide anion radical scavenging activities of SPH are dependent on enzymes rather than DH.
Acknowledgments
The authors thank the Dean, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi for providing the necessary facilities to undertake this work.
Funding
Financial support was given by the Department of Science and Technology (DST), Government of India (GOI), New Delhi to carry out this research work.