Optimal Immobilization of Acidic Proteases from Monterey Sardine (Sardinops sagax caeurelea) on Partially Deacetylated Chitin from Shrimp Head Waste

ABSTRACT

Response surface methodology was employed to optimize the immobilization yield of acidic proteases from Monterey sardine (Sardinops sagax caeurelea) using partially deacetylated chitin as immobilization support. A rotatable central composite design was applied to evaluate the effects of immobilization conditions such as enzyme loading (X₁), immobilization pH (X₂), and tripolyphosphate concentration (X₃) on the immobilization yield. The analysis of variance revealed that the established model was significant (p < 0.05), and the adjustment of the quadratic model with the experimental data was satisfactory. Under optimal conditions (X₁ = 0.05 mg/mL, X₂ = 3.16, and X₃ = 0.75%), an immobilization yield of 79.1% was achieved; a value that was in accordance with the predicted one.

KEYWORDS:

• Response surface methodology
• acidic proteases
• immobilization
• chitin