Tandem Mass Tag-Based Proteomics Reveals the effect of Electron Beam Irradiation on Metabolism-Related Differentially Expressed Proteins in Solenocera melantho Postmortem

ABSTRACT

Postmortem metabolism in shrimp is an important factor affecting muscle quality during its preservation. Electron beam irradiation (EBI) can effectively kill microorganisms and inactivate enzymes. In this study, the tandem mass tag (TMT) method was used to study the changes in the muscle metabolism of Solenocera melantho postmortem before and after EBI (6 kGy). The TMT method identified a total of 656 proteins, including 120 differentially expressed proteins (DEPs) (56 upregulated, 64 downregulated). The analysis of these results showed that EBI can inactivate most enzymes and slow down their corresponding metabolic processes. These results also showed that irradiation can denature some proteins. The study revealed the effects of EBI on the muscle quality of S. melantho at the molecular level.

KEYWORDS:

• Solenocera melantho
• TMT
• proteomics
• electron beam irradiation
• metabolism

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplemental data

Supplemental data for this article can be accessed online at https://doi.org/10.1080/10498850.2021.2010852

Additional information

Funding

This work was supported by National Key R&D Program of China (2020YFD0900903), Research on the key technology of fresh agricultural products storage and transportation logistics—the research and industrialization demonstration of the first-line fresh-keeping and cold chain logistics technology of sea-caught shrimp (2019C02086), and Industrial chain Collaborative Innovation Project of the Demonstration Work on Innovative Development of The Marine Economy of the State Oceanic Administration (NBHY-2017-S2)