ABSTRACT
The sea cucumber ethanol extracts (SCEEs) and protein hydrolysates (SCPHs) of Holothuria atra(HA), H. leucospilota(HL), and Bohadschia marmorata (BM) were prepared sequentially using 60% ethanol and Neutrase. The SCEE of HL contained phenolic acids (4.63 ± 0.50 µg GAE/mg extract) and was the most potent radical scavenger (91.76 ± 0.76%) at 10 mg/mL; whereas its SCPH contained hydrophobic amino acids (31.25 g/100 g) and inhibited glycation by 86.16 ± 5.92% at 5 mg/mL. Furthermore, the SCEE of HA inhibited tyrosinase by 49.93 ± 0.27% at 10 mg/mL, related to sulfated saponins (121.82 ± 3.52 µg xylose/mg extract). This study reports SCEEs as antityrosinase and SCPHs as antiglycation agents for the first time.
Acknowledgments
This research was funded by the Ministry of Marine Affairs and Fisheries (Grant Number SP DIPA-032.12.2.403835/2018). The authors thank B. R. Simanjuntak for his assistance with sampling collection and radical scavenging assay. We also acknowledge N. N. Solihat for her help with LC-MS analysis.
Disclosure statement
No potential conflict of interest was reported by the authors.
Supplementary material
Supplemental data for this article can be accessed online at https://doi.org/10.1080/10498850.2022.2163862