Development of combined medium for in-vitro co-culture of mesenchymal stem cell and pseudomonas aeruginosa

ABSTRACT

A co-culture of cells, bacteria and biomaterial could effectively test the performance of antibacterial biomaterials in infection states that mimic actual clinical conditions. As cultivating environments of cells and bacteria vary in many aspects, the development of a suitable co-culture medium is required for the co-culturing of bacteria and cells. The aim of this study was to develop a suitable combined medium for co-culturing of human bone marrow-derived mesenchymal stem cells (BMSCs) and Pseudomonas aeruginosa (P. aeruginosa). Combined medium at different ratios of Dulbecco’s modified eagle medium containing 10% fetal bovine serum (D10) and mueller hinton broth were prepared. From the evaluation of cell morphology, growth, viability, bone differentiation, and bacteria growth, the ratio of 75% D10 and 25% mueller hinton broth was suggested to be favourable in supporting the co-culturing of BMSCs and P. aeruginosa.

KEYWORDS:

• Co-culture
• mesenchymal stem cell
• combined medium
• pseudomonas aeruginosa

Acknowledgments

This research is supported by the Singapore Ministry of Health’s National Medical Research Council under its NMRC Open Fund Individual Research Grant Number OFIRG15nov026

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Singapore Ministry of Health’s National Medical Research Council [NMRC Open Fund Individual Research Grant Number OFIRG15nov026]