Intravascular Circulation and Distribution of Human ⁵¹Cr-DBBF Stroma-Free Hemoglobin, ⁵¹Cr-Plasma, ⁵¹Cr-Saline, ⁵⁹Fe-Plasma, and ¹²⁵I-Albumin in the Mouse

Abstract

Male B6C3HF1 mice were infused with human ⁵¹Cr-labeled DBBF (bis 3,5-dibromosalicyl fumarate) crosslinked stroma-free hemoglobin (SFH). In the first hour following SFH infusion, 11.2% of the infused radioactivity was found in the skin, 11.4% in muscle, 9.1% in the skeleton, and 5% in the liver. Twenty-four hours after infusion, 15.4% of the radioactivity was found in the skin, 10.3% in the muscle, 16.6% in the skeleton, and 6.7% in the liver.

The circulation and distribution of ⁵¹Cr-labeled DBBF-SFH were compared with levels of ⁵¹Cr labeled plasma, ⁵¹Cr in saline, ⁵⁹Fe labeled plasma, and ¹²⁵I albumin. The radioactivity in the blood was similar for ⁵¹Cr-DBBF-SFH, ⁵¹Cr-plasma, and ⁵⁹Fe-plasMA During the 24-hour post-infusion period, extravascular distribution of the ⁵¹Cr-saline, ⁵¹Cr-plasma, and ¹²⁵I albumin within the organs was similar to that of ⁵¹Cr-DBBF-SFH, with the highest levels being in skin, muscle, skeleton and liver, and no increase in the levels in the lung or spleen. The distribution of ⁵⁹Fe compared to that of ⁵¹Cr-DBBF, ⁵¹Cr-plasma, ⁵¹Cr-saline, and ¹²⁵I albumin can be explained by the fact that ⁵⁹Fe is utilized in the production of new red blood cells.