Analytical Peptide Mapping of a Complex Yellowfin Tuna Myoglobin Peptic Hydrolysate by High Performance Liquid Chromatography

Abstract

A method is described for a real identification of any peptides isolated from a complex peptic Yellowfin tuna red muscle myoglobin hydrolysate. A combination of size exclusion and reversed phase high performance liquid chromatography have proved to be a useful strategy for fractionation of such a mixture. This technique enable a large number of pure peptides from the total hydrolysate to be obtained. Peptides were identified and located on the known myoglobin sequence from their amino acid content determined by the Pico-Tag method and a second order derivative spectroscopic method. Location of the peptides allowed us to define effective cut sites of the porcine pepsin. The procedure described in this study will be useful for acquiring a better knowledge of such an hydrolysate.