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Abstract
In this study in vitro inhibition of the motility parameters (motility ‐ higher than 10 μm/s; progressive movement ‐ higher than 50 μm/s; path velocity; straightness; mean pixel count as well as mean intensity) of bovine spermatozoa by cadmium chloride in four doses (0.02; 0.1; 0.2; and 2.0 mg CdCl2/ml natrium citrate) evaluated by computerized semen analyzer HTM is reported.
Decreasing time‐dependent tendency of progressive movement was evident in control group as well as in all cadmium treated groups except the group with the highest concentration of cadmium (2.0 mg CdCl2/ml), where almost all parameters were expressively affected. In control group average time ‐ dependent decrease (Time 0/1) in progressive movement was 23.6% (30.4/6.8), in group with 0.02 mg CdCl2/ml 31.4% (34.4/3.0), in group with 0.1 mg CdCl2/ml 21.8% (25.6/ 3.8), in group with 0.2 mg CdCl2/ml 42.8% (44.0/1.2). Due to the very low value of progressive movement at time 0 and time 1 difference in group with 2.0 mg CdCl2/ml was only 4.57% (5.17/0.6).
Differences in motility were similar in all groups and time periods (86.0 ‐ 96.0%), certain decrease was in group with cadmium concentration 0.2 mg/ml at time 1 (65.8%) and in the group with cadmium concentration 2.0 mg/ml at time 0 (74.33%) and at time 1 (31.4%). Also path velocity and straightness were mostly affected in the group with the highest cadmium concentration.
