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A centnfugation method was developed to determine water holding capacity (WHC) of lignin, cellulose, pectin, whole locust bean gum (LBGW) and insoluble locust bean gum (LBGI) and was compared with the AACC method using cellulose, lignin and LBGI. WHC was found to be highest for LBGW>pectin>LBGI>lignin>cellulose. Solubility and chemical configuration rather than particle size, were the main determinants of WHC. The AACC method tended to overestimate WHC for cellulose, LBGI and lignin.
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