Effect of Sample Solubilized Aliquot Volume and Concentration on Hydroperoxide Detection in PUFA-Rich Fish Oil

Abstract

The objective of this work was to study the effect of sample oil concentration and volume on hydroperoxide determination in n-3 PUFA-rich sardine-anchovy oil. Oil samples were stored at −20°C, 6 d, air-free (T1); and 20°C, 2 d in air (T2). Significant differences between storage conditions were observed on hydroperoxide concentration. High reproducibility was obtained even in sample concentrations as low as 5 mg oil/mL of propan-1-ol. A high correlation was observed between the modified ferrous oxidation-xylenol orange method (FOX2) and AOAC peroxide value analysis methods, although FOX2 was faster, more sensitive, and precise when analyzing small hydroperoxide concentrations.

Keywords:

• Fish oil
• Lipid oxidation
• n-3 Fatty acids
• Hydroperoxides

ACKNOWLEDGMENTS

Violeta Ugalde-Benítez wishes to thank the National Council of Science and Technology (CONACYT, Mexico) for a graduate scholarship.