Laundry detergent-stable lipase from Pacific white shrimp (Litopenaeus vannamei) hepatopancreas: Effect of extraction media and biochemical characterization

ABSTRACT

Extraction and biochemical properties of a new lipase from the hepatopancreas of Pacific white shrimp were studied. Recovery of the hepatopancreas powder with 50 mM Tris-HCl, pH 7.0 containing 0.2% (v/v) Brij35 gave a higher recovery of lipase activity than other extractants tested (p < 0.05). The optimal pH and temperature for lipase activity were 8.5 and 60°C, respectively, when p-nitrophenyl palmitate was used as a substrate. The enzyme was stable to heat treatment up to 40°C and over a pH range of 7.0–10.0 for 30–120 min. Lipase activities continuously decreased as the sodium deoxycholate (NaDC) concentration increased, but activities increased as NaCl concentration increased up to 3.0 M. Hydrolytic activity was enhanced by NaN₃, but strongly inhibited by Hg²⁺, Cu²⁺, Al³⁺, and phenylmethanesulfonyl fluoride. The lipase was evaluated as highly stable against surfactants (Tween 20, Tween 80, Triton X-100, and gum arabic). However, the enzyme was unstable against sodium dodecyl sulphate. Stability of the lipase with commercial liquid and solid detergents (Attack^®, Bres^®, Omo^®, and Pao^®) was also investigated. The lipase exhibited substantial stability and compatibility with tested commercial liquid and solid laundry detergents for 30–60 min. The overall properties of the lipase from Pacific white shrimp hepatopancreas, thus leading us to propose that it is an excellent candidate for use as biocatalysts for better detergent formulation.

KEYWORDS:

• Characterization
• Lipase
• Viscera
• Shrimp, Detergent

Conclusions

The hepatopancreas of Pacific white shrimp would be a potential source of lipase for certain food processing operations that require high alkaline and high salt condition. In addition, considering the high activity and stability in high alkaline pH, relative stability in the presence of surfactants and several commercial laundry detergents, Pacific white shrimp hepatopancres lipase may find application in laundry detergents.

Funding

This research was supported by the Thailand Research Fund (TRF) and Thaksin University. The TRF distinguished research professor grant was also acknowledged.

Additional information

Funding

This research was supported by the Thailand Research Fund (TRF) and Thaksin University. The TRF distinguished research professor grant was also acknowledged.