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Original Articles

Pharmacological activities of brown seaweed Sargassum wightii (Family Sargassaceae) using different in vitro models

, &
Pages 931-945 | Received 05 Feb 2016, Accepted 10 May 2016, Published online: 24 Oct 2016
 

ABSTRACT

Recently a great deal of interest has been developed towards natural bioactive components from seaweeds as functional food ingredients. Antioxidant, anti-inflammatory, antidiabetic, and antihypertensive activities of ethyl acetate:methanol and chloroform solvent fractions of the brown seaweed Sargassum wightii were evaluated using different in vitro systems. The ethyl acetate:methanol fraction registered greater Fe2+ ion chelating ability (IC50 0.52 mg/ml), and were effective in stabilizing the 2,2-azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (IC50 0.82 mg/mL), and 1,1-diphenyl-2-picryl hydrazil radicals (IC50 0.32 mg/mL) than those derived from chloroform solvent fractions. The chloroform solvent fraction showed greater angiotensin converting enzyme-I inhibitory activity (IC50 0.084 mg/mL), while the ethyl acetate:methanol fraction exhibited greater anti-COX-1, 2, and 5-LOX (IC50 0.03-0.05 mg/mL) and DPP-4 inhibitory (IC50 ~ 0.013 mg/mL) properties. A significant co-linearity was recorded between target bioactive properties and the electronegative groups appeared in the downfield space of the nuclear magnetic resonance spectra of the ethyl acetate:methanol and chloroform solvent fractions from S. wightii.

Nomenclature

COX-1=

Cycloxygenase-1

COX-2=

cycloxygenase-2

5-LOX=

5-liopxygenase

DPP-4=

dipeptidyl peptidase-4

ACE=

angiotensin-I converting enzyme

NMR=

nuclear magnetic resonance

ABTS=

2, 2′-azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium salt

DPPH=

1, 1-diphenyl-2-picryl-hydrazil

PCA=

principle component analysis

TBARS=

thiobarbituric acid reactive species

Acknowledgments

The authors thank the Director, Indian Council of Agricultural Research-Central Marine Fisheries Research Institute (ICAR-CMFRI), for his guidance and support. Thanks are due to the Head, Marine Biotechnology Division for facilitating the research works.

Funding

This work was supported by the funding under the Science & Engineering Research Board (SERB) Scheme (Grant number SR/S1/OC-96/2012 SERB) from Department of Science and Technology, Ministry of Science and Technology, New Delhi, India. Anusree Maneesh acknowledges ICAR, for the award of a scholarship. Fasina Makkar would like to thank the Department of Science and Technology for fellowship.

Conflict of interest

The authors declare that there is no conflict of interest.

Additional information

Funding

This work was supported by the funding under the Science & Engineering Research Board (SERB) Scheme (Grant number SR/S1/OC-96/2012 SERB) from Department of Science and Technology, Ministry of Science and Technology, New Delhi, India. Anusree Maneesh acknowledges ICAR, for the award of a scholarship. Fasina Makkar would like to thank the Department of Science and Technology for fellowship.

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