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ABSTRACT
The purpose of the study was to determine if pomegranate (Punica granatum L.) peel contains polyphenolic compounds with antibacterial activity against Cronobacter sakazakii. Minimum inhibitory and bactericidal concentrations of a methanolic extract were found to increase significantly (P < .05) with decreasing temperature (37, 21, 10 °C) and pH (6.0, 5.0, 4.0). Chemical analysis by high pressure liquid chromatography revealed that the elligitannins α- and β-punicalagin were most abundant hydrolysable polyphenolic compounds in the extract, followed by ellagic acid, ellagic acid derivatives and punicalin. Pomegranate peel is a potential source of natural preservatives for the control of Cronobacter sakazakii in food.
Acknowledgments
The lead author is grateful to the Sakarya University Scientific Research Projects Unit for the scholarship which supported this study. The authors wish to thank Oktay Yemiş, Kevin Usher, Steve Orban, and John Drover for valuable technical assistance and suggestions throughout this study.