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RESEARCH PAPERS
Food Science and Technology Section

CARACTERIZACIÓN PARCIAL DEL COLÁGENO EXTRAÍDO A PARTIR DEL MANTO, ALETA Y TENTÁCULOS DE CALAMAR GIGANTE (Dosidicus gigas)
PARTIAL CHARACTERIZATION OF COLLAGEN FROM MANTLE, FIN, AND ARMS OF JUMBO SQUID (Dosidicus gigas)

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Pages 101-108 | Received 05 Feb 2008, Accepted 29 May 2008, Published online: 02 Oct 2009
 

Abstract

Resumen

In Dosidicus gigas or jumbo squid, collagen plays an important role in squid swimming mechanisms. Scarce information is available on collagen. The content as well some physical and chemical properties of pepsin-soluble (PSC) and insoluble collagen (IC) were compared in mantle, arm, and fin from adult fresh jumbo squid. The total collagen content and SDS-electrophoresis profile were different in each anatomic part evaluated, the highest IC value was in arms. Scanning electron microscopy (SEM) studies showed same structural arrangement in the PSC from the three anatomical parts and different structural arrangement in the IC. DSC showed a very high transition at 115–120°C for PSC. The highest Tmax and ΔH was detected in arms collagen. This knowledge will aid to acquire a better understanding and to improve the commercial application of this economically important commodity.

El colágeno en el Dosidicus gigas o calamar gigante participa en los mecanismos natatorios del organismo. Sin embargo, la información fisicoquímica sobre esta proteína es escasa. En este estudio se comparó el contenido y algunas propiedades fisicoquímicas de colágeno soluble en pepsina (CSP) e insoluble (CI) extraídos de manto, aleta y tentáculos de Dosidicus gigas fresco. El contenido de colágeno total, así como el perfil electroforético fueron diferentes para cada región anatómica evaluada. Los tentáculos presentaron el mayor contenido de CI. En estudios de microscopia electrónica de barrido (MEB) se observó un arreglo estructural similar en el CSP de las tres regiones y en el CI, marcadas diferencias en el empaquetamiento de las fibras. Las tres fracciones proteicas de colágeno, mostraron una alta resistencia térmica (115–120°C), siendo superior para tentáculos en comparación con aleta y manto. Esta información contribuye a un mayor conocimiento de la especie y una mejor aplicación comercial de la misma.

Palabras clave: Calamar, colágeno, manto, aleta, tentáculos

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