Superoxide Dismutase Enzymes in Oxidative Type Yeast H. Polymorpha

ABSTRACT

Hansenula polymorpha CBS 4732, cultivated on media containing glucose, glycerol or methanol as carbon source has been investigated for specific activity of Superoxide dismutase (SOD) enzymes during batch—wise cultivation. Furthermore cell free extract from the analyzed strain cultivated on different carbon sources were subjected to 8% native PAGE. One band corresponding to sensitive of KCN Cu/Zn SOD with Rm 0.29 is inhibiting in all samples. After inhibition of cyanide sensitive Cu/Zn SOD, three bands specifically stained for SOD activity, corresponding to Mn SOD are observed in samples obtained by H. polymorpha cultivated on medium containing methanol. Two bans are retaining in glucose and glycerol grown cells. This data suggest that probably in H. polymorpha yeasts appears multiple enzyme forms of Mn SOD enzyme. Multiple enzyme forms become more stable and do not disappear after 1 min at 75°C. One band with Rm 0.34 in all yeast samples cultivated on methanol, glucose and glycerol, remains till heating at 75°C for 10 minutes.

Keywords:

• enzyme forms
• Hansenula polymorpha
• superoxide dismutase activity