Association of P2X7R gene with serum lipid profiles in Chinese postmenopausal women with osteoporosis

Abstract

Objectives: The aim was to study the relationship between purinergic P2X7 receptor (P2X7R) functional genetic polymorphisms and lipid profiles in Chinese postmenopausal osteoporosis (OP) patients and controls.

Methods: A total of 517 OP patients and 531 controls were randomly recruited by cluster sampling; blood was drawn to analyze P2X7R genetic polymorphisms and serum lipid levels. Bone mineral density (BMD) at the lumbar spine and hip was measured via dual-energy X-ray absorptiometry.

Results: P2X7R rs2230911 was found to be associated with serum total cholesterol, triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein, and BMD at the lumbar spine and hip in OP patients (p < 0.05). P2X7R rs3751143 was shown to be associated with serum TG and hip BMD in OP patients (p < 0.05). Furthermore, their estimated haplotype (rs1718119G-rs2230911C-rs3751143A) was indicated to be associated with TG, HDL, and BMD at the lumbar spine and hip in OP patients. However, no difference in the level of lipid profiles or BMD was found to be related to P2X7R genotype or haplotype in the control group (p>0.05).

Conclusions: Our findings suggest that P2X7R functional genetic polymorphisms and their estimated haplotypes are associated with serum lipid concentrations and BMD among Chinese postmenopausal women with OP.

Chinese abstract

目的：探讨绝经后骨质疏松症（OP）患者和对照组嘌呤能P2X7受体（P2X7R）功能基因多态性与血脂的关系。

方法：随机抽取517例OP患者和531例对照组, 抽取血液, 分析P2X7R基因多态性和血脂水平。应用双能X线骨密度仪测量腰椎和髋部骨密度。

结果：发现P2X7R rs2230911与血清总胆固醇、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白及OP患者的腰椎和髋部骨密度有关（P < 0.05）。P2X7R rs3751143与血清TG和OP患者的髋部骨密度相关（P < 0.05）。此外, 上述基因估计的单倍型（rs1718119g-rs2230911c-rs3751143a）表明与OP患者腰椎和髋部的TG、HDL和BMD相关。然而, 在对照组中, 脂质分布或BMD水平的差异与P2x7r基因型或单倍型无关（p > 0.05）。

结论：我们的研究结果表明, 在中国绝经后OP妇女中, PX7R功能基因多态性及其估计单倍型与血脂和骨密度相关。

Keywords:

• Lipid
• osteoporosis
• polymorphism
• P2X7 receptor
• postmenopausal women

Acknowledgements

The authors thank all of the study subjects for volunteering to participate in the study.

Potential conflict of interest

The authors report no conflicts of interest.

Additional information

Funding

This work was supported by grants from National Natural Science Foundation of China [NO. 81760595], [NO. 81560529], [NO. 81302501]; Natural Science Foundation of Jiangxi Province [NO. 20171ACB21003], [NO. 20181BAB205068], [NO. 20161BAB205209]; Outstanding Young Scientist Training Object of Jiangxi Province [NO. 20162BCB23021]; Technology Pedestal and Society Development Project of Jiangxi Province [NO. 20151BBG70249]; China Scholarship Council Foundation [201806825027]; Nanchang University Students’ innovation and entrepreneurship training program [NO. 201710403039], [NO. 201810403082], [NO. CX2018181], [NO. 2017247], [NO. 2016211].