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Natural Product Research
Formerly Natural Product Letters
Volume 33, 2019 - Issue 5
172
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Short Communication

Effect of Agave americana L. on the human, and Aspergillus oryzae S2 α-amylase inhibitions

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Pages 755-758 | Received 22 Aug 2017, Accepted 07 Nov 2017, Published online: 28 Nov 2017
 

Abstract

Among phenolic compounds, Agave americana L. extract contained puerarin (38.4%) and p-coumaric acid (12.29%) (pCa). From the Lineweaver–Burk plots, pCa and puerarin demonstrated a competitive and a non competitive inhibitions towards human α-amylase activity, respectively. PCa exhibited a higher human inhibitory activity with an IC50 of 98.8 μM which was about 2.3 times than acarbose. Puerarin (IC50 = 3.87 μM) and pCa (IC50 = 10.16 μM) also showed an excellent inhibition for Aspergillus oryzae S2 α-amylase activity. The inhibitions of the described biocatalysts compounds towards both amylases were significantly decreased when they were pre-incubated with starch. The binding modes of these compounds were evaluated in silico. The binding efficiency order of these molecules in terms of polar contact numbers for both enzymes was in agreement with the in vitro studies. These findings provided a rational reason to establish the isolated compounds capability as therapeutic target for hyperglycaemia modulation and antifungal therapy.

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