Identification of Differentially Expressed Proteins in Cassava Infected with Colletotrichum gloeosporioides f. sp. manihotis

Abstract

Cassava anthracnose disease (CAD), caused by Colletotrichum gloeosporioides f. sp. manihotis infection, is a major disease of cassava (Manihot esculenta Crantz). The objective of this study was to identify proteins differentially regulated in resistant (‘Huaybong 60’) and susceptible (‘Hanatee’) cultivars in response to C. gloeosporioides f. sp. manihotis infection. Total proteins were extracted and resolved via 2-dimensional gel electrophoresis. Fourteen differentially expressed proteins were identified in ‘Huaybong 60’, of which eight were up-regulated and seven down-regulated. In ‘Hanatee’, seven proteins were identified, of which one was up-regulated and six were down-regulated. Proteins associated with carbohydrate metabolism were down-regulated, whereas reactive oxygen species-generating and cyanogenic pathways were up-regulated in the resistant cultivar. This study provided new insights into the mechanisms underlying pathogen resistance in cassava and suggested that pathogen resistance might arise from an integrated mechanism that arises from only a few initiating events.

KEYWORDS:

• 2-DE
• cassava
• cassava anthracnose disease
• Colletotrichum gloeosporioides f. sp. manihotis
• Manihot esculenta

Additional information

Funding

This research was supported by the National Center for Genetic Engineering and Biotechnology (Thailand), the Thailand Research Fund, and Mahidol University. Ratchadaporn Thaikert was fully supported for Ph.D. study by the Commission on Higher Education, Ministry of Education, Thailand.