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Research Article

Glucose-6-phosphate dehydrogenase deficiency screening and gene analysis in blood donors of Guangdong province

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ABSTRACT

Objectives

The characteristic of glucose-6-phosphate dehydrogenase (G6PD) deficiency is red blood cell (RBC) destruction in response to oxidative stress. Patients requiring RBC transfusions may simultaneously receive oxidative medications or have concurrent infections, both of which can induce hemolysis in G6PD-deficient RBCs. We intend to investigate the incidence of G6PD deficiency in voluntary blood donors and to evaluate the transfusion risk associated with G6PD deficiency in Guangdong province.

Methods

G6PD enzyme was analyzed in 3042 donors and gene mutations were genotyped in G6PD-deficient samples.

Results

The G6PD-deficient prevalence of voluntary blood donors was 6.97% (212/3042), 55.19% blood donors with G6PD deficiency donated blood more than twice. Eighty-five cases of G6PD deficiency were genotyped, and the common types of G6PD mutations were c.1376 G > T, c.1388 G > A, c.95 A > G, c.1024 C > T, and c.871 G > A.

Conclusions

Due to the high prevalence of G6PD deficiency in Foshan area, we recommended that the screening of G6PD deficiency should be carried out for the regular blood donors to ensure the safety of blood users.

Acknowledgements

We thank the research assistants and all blood donors who took part in this study. Author contributions: L-YY conceived and designed the study and manuscript revision. H-FL, FL, JL, J-RW, and Z-XC performed the experiments. H-FL, FL, and L-YY analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

We would like to thank Natural Science Foundation of Guangdong Province [grant number 2016A030307035] and High Level Development Plan of People’s Hospital of Yangjiang [grant number G2020007] for their financial support.