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Abstract
A high frequency and rapid regeneration protocol was developed from leaf and nodal explants of Enicostemma littorale Blume on MS medium supplemented with 6-benzyl amino purine (BAP; 3 mg/l) and naphthalene acetic acid (NAA; 1 mg/l). Maximum number of shoots was obtained on medium containing BAP. The regenerated shoots were further elongated on the medium having GA3. In vitro shoots were then excised and transferred to rooting medium containing IAA or IBA (0.5–1.0 mg/l). The rooted plantlets were hardened in polycups containing sterile soil and vermiculite (1:1). Plantlets, thus obtained were finally transferred to a greenhouse. The plantlets showed high survival rate (80%) in the soil.