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Abstract
In this study, we isolated a homozygous T-DNA insertion mutant line, ckl2, of the casein kinase 1-Like 2 (CKL2) gene in Arabidopsis thaliana. Through analysis of the germination ratio, root length, water loss rate, and stomatal aperture, we found that the ckl2 mutants showed an abscisic acid (ABA)-hyposensitive phenotype in all tests. CKL2 was also found to mediate the expression of ABA-upregulated genes in seeds and seedlings. CKL2 was expressed in all tissues, especially in the leaf and leaf stalk, and could be induced by ABA. A proline accumulation experiment showed that the Pro content in ckl2 was lower than that of the wild type. The above results demonstrate that CKL2 was required for ABA-regulated seed germination, root growth, and gene expression, suggesting that CKL2 positively mediates ABA signaling in Arabidopsis.
Acknowledgements
This research was supported by grants from the National Natural Science Foundation of China (31071076 and 30871325), the Program for New Century Excellent Talents in University (NCET-10-0363 to X. Guo), the Excellent Youth Foundation of Hunan Province (11JJ1005), the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, the State Education Ministry ([2011]1139 to X. Guo), and the SIT Project of Hunan University, 2012 and 2013.
