![Sample our Environment & Agriculture journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5934/TOC-Subject-Sample-2021-Environment-Agriculture.jpg"})
Abstract
The changes in accumulation of two potential osmoprotectants (proline and glycine betaine), lipid peroxidation appraised as malondialdehyde (MDA) level, activities of key antioxidant enzymes such as superoxide dismutase (SOD: EC 1.15.1.1), catalase (CAT: EC 1.11.1.6), peroxidase (POD: EC 1.11.1.7), and glutathione reductase (GR: EC 1.6.4.2), and soluble protein profile in two cultivars of mulberry (S146 and Sujanpuri) differing in alkalinity (NaHCO3) tolerance were investigated at 2-month intervals up to 6-month growth under stress conditions. Varying levels of salinity–alkalinity developed in soil were 0, 30, 40, and 50 g of NaHCO3 kg−1 soil with pH 7.8, 9.1, 9.8, and 10.3, respectively. Alkali stress led to a consistent accumulation of proline and glycine betaine in mulberry leaves with time. The activities of leaf SOD, CAT, POD, and GR increased with increase in external salt concentration and pH. The increase in antioxidant enzyme activities was higher in cv. S146 than cv. Sujanpuri, whereas rate of lipid peroxidation measured in terms of MDA was higher in cv. Sujanpuri as compared to cv. S146. Protein profile revealed that some unknown proteins of low molecular mass (10–32.5 kDa) were induced by NaHCO3 stress, but differently in two cultivars.
| Abbreviations | ||
| ROS | = | reactive oxygen species |
| GB | = | glycine betaine |
| MDA | = | malondialdehyde |
| SOD | = | superoxide dismutase |
| CAT | = | catalase |
| POD | = | peroxidase |
| GR | = | glutathione reductase |
| Abbreviations | ||
| ROS | = | reactive oxygen species |
| GB | = | glycine betaine |
| MDA | = | malondialdehyde |
| SOD | = | superoxide dismutase |
| CAT | = | catalase |
| POD | = | peroxidase |
| GR | = | glutathione reductase |
Acknowledgments
This paper is part of PhD work of Dr Parvaiz Ahmad. S Sharma was the PhD guide of Dr Parvaiz Ahmad. Prof. S Gucel, cross checked the results which Dr Parvaiz got during the work on this paper and helped him a lot in writing this paper. Prof. Munir Ozturk, drafted the experimental design, which cultivars we should use and also helped Dr Parvaiz in statistical analysis of this paper. Authors are thankful to CSIR-New Delhi, India for providing financial assistance to PA.
