Comparative transcriptome analysis of the resistance mechanism of Hemerocallis citrina Baroni to Puccinia hemerocallidis infection

ABSTRACT

Hemerocallis citrina Baroni (daylily) is an important cash crop in many regions of China. Daylily rust caused by Puccinia hemerocallidis (Ph) is a major disease in H. citrina. Rust infection on the leaves of two resistant and susceptible varieties of H. citrina was examined. Transcriptome sequencing and defense enzyme activity were used to analyze the differential expression of related genes after 0, 72, 144, 192, and 288 h, to reveal the response mechanism of H. citrina to rust infection and screen-related resistance genes. The results suggested that 72 h after Ph infection was the key time point for determining differences between resistant and susceptible varieties. DEGs were significantly enriched in phenylpropanoid biosynthesis, plant–pathogen interaction, plant hormone signal transduction, and the MAPK signaling pathway. TFs WRKY33 and CYP98A are key genes closely related to the disease-resistance response of H. citrina. This study is the first large-scale transcriptomic analysis of H. citrina.

KEYWORDS:

• Daylily
• enzymatic activities
• RNA-seq
• transcription factors
• resistance genes

Acknowledgements

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript. Conceptualization, methodology, validation, formal analysis, writing – original draft preparation, L.J.Z.; investigation, J.M.; resources, S.W.; data curation, S.L. and N.Y.; visualization, F.T.; writing – review and editing, supervision, project administration, funding acquisition, L.L.Z and X.Y. All authors have read and agreed to the published version of the manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The raw data were deposited in the National Centre for Biotechnology Information(NCBI) Sequence Read Archive (SRA) database with accession number PRJNA891118.

Additional information

Funding

This research was funded by the Suqian Science and Technology Program [K201921], the Suqian Agricultural Science and Technology Independent Innovation Project [SQCX202209].

Notes on contributors

Lijie Zhang

Lijie Zhang holds a degree in Biological Sciences from Anhui Institute of Science and Technology, China (2006) and a master's in Microbiology from Fujian Agriculture and Forestry University, China (2009). She is currently an associate professor at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. She has experience in the area of microbiology, molecular biology, plant pathology and plant breeding, working mainly on the following topics: plant and microbial interactions, transcriptomics and Breeding of disease-resistant daylily varieties.

Lingling Zhou

Lingling Zhou holds a degree in Agronomy from Shenyang agricultural university, China (2007) and a master's in Agronomy from Nanjing Agricultural University, China (2010). She is currently an associate professor at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. She has experience in the area of microbiology, molecular biology and plant breeding, working mainly on the following topics: breeding and cultivation techniques of daylily varieties.

Jiali Meng

Jiali Meng holds a degree in Agronomy from Nanjing Agricultural University, China (2009) and a master's in Agronomy from Nanjing Agricultural University, China (2012). She is currently a research associate at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. She has experience in the area of molecular biology, proteomics and plant breeding, working mainly on the following topics: cold tolerance of vegetable, plant grafting technique, proteomic analysis and breeding of low temperature tolerant varieties.

Shaojun Wu

Shaojun Wu holds a degree in Agronomy from Yunnan Agricultural University, China (2008) and a master's in Agronomy from Nanjing Agricultural University, China (2011). He is currently a research associate at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. He has experience in the area of vegetable cultivation, plant physiology and vegetable breeding, working mainly on the following topics: vegetable breeding technology, vegetable continuous cropping obstacle prevention technology, vegetable and disease interaction.

Shuhua Liu

Shuhua Liu holds a degree in Agronomy from Anhui Institute of Science and Technology, China (2017) and a master's in Agronomy from Nanjing Agricultural University, China (2020). He is currently a research assistant at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. He has experience in the area of plant nutrition and plant physiology, working mainly on the following topics: crop growing, plant and disease interaction.

Nianfu Yang

Nianfu Yang holds a degree in Agronomy from Anhui Agricultural University, China (2017), and a master's in Agronomy from Nanjing Agricultural University, China (2020). He is currently a research assistant at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. He has experience in the area of Vegetable science, vegetable breeding for disease resistance, vegetable cultivation, working mainly on the following topics: breeding of vegetable resistance varieties, high efficiency and high quality cultivation technology.

Fufa Tian

Fufa Tian holds a degree in Vegetable Science from Qingdao Agricultural University, China (2000) and a master's in Vegetable Science from Huazhong Agricultural University, China (2003). He is currently an associate professor at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. He has experience in the area of Vegetable culture, vegetable breeding and plant pathology, working mainly on the following topics: vegetable breeding, Vegetable cultivation and disease control technology.

Xiang Yu

Xiang Yu holds a degree in Agronomy from Nanjing Agricultural University, China (2005) and a master's in Agronomy from Nanjing Agricultural University, China (2008). He is currently an associate professor at Institute of Suqian, Jiangsu Academy of Agricultural Sciences. He has experience in the area of vegetable culture, vegetable breeding science and molecular biology, plant pathology and plant breeding, working mainly on the following topics: breeding of high yield and high quality vegetable varieties, efficient fertilization technology and improvement of vegetable quality.