Efficient synthesis of vitamin A palmitate in nonaqueous medium using self-assembled lipase TLL@apatite hybrid nanoflowers by mimetic biomineralization

ABSTRACT

To conveniently prepare the immobilized enzyme for green synthesis of vitamin A palmitate, thermomyces lanuginosus lipase (TLL) was assembled with apatite derived from calcium phosphate in solution to form TLL@apatite hybrid nanoflowers (hNFs) by mimetic biomineralization. The obtained hNFs was characterized by SEM and applied in the enzymatic synthesis of vitamin A palmitate. Under optimal condition, when palmitic acid and vitamin A acetate were used as substrates, the yield of the target product was up to 90.4%, about 1.5 folds of that using free TLL. In addition, when each cycle for catalytic synthesis was carried out in petroleum ether with 20% water content as solvent for 12 h, the hNFs still retains 65.8% of initial catalytic synthetic ability after 10 cycles. These results demonstrated the hNFs was a kind of green biocatalyst with high catalytic activity and high stability with great potential in the green pharmaceuticals industry.

GRAPHICAL ABSTRACT

KEYWORDS:

• Biomineralization
• vitamin A palmitate
• hybrid nanoflowers
• thermomyces lanuginosus lipase
• green pharmaceuticals

Disclosure statement

No potential conflict of interest was reported by the authors.

Notes on contributors

Liqing Xu is studying in College of Materials, Chemistry and Chemical Engineering (Hangzhou Normal University, Hangzhou, China). Her research has focused on the biocatalysis and biotransformation.

Jianyun Yu, female, born in 1992, is studying as a Master candidate in College of Materials, Chemistry and Chemical Engineering (Hangzhou Normal University, Hangzhou, China). Her research is focused on the enzyme immobilization.

Anming Wang, male, born in 1975, associated professor. He is working in College of Materials, Chemistry and Chemical Engineering (Hangzhou Normal University, Hangzhou, China). His research interest is biocatalysis and green chemical engineering, mainly engaged in the enzyme immobilization and its catalysis application in the synthesis and development of chiral drugs.

Chengyi Zuo is studying in College of Materials, Chemistry and Chemical Engineering (Hangzhou Normal University, Hangzhou, China). Her research has focused on the biocatalysis and biotransformation.

Huimin Li is studying as a Master candidate in College of Materials, Chemistry and Chemical Engineering (Hangzhou Normal University, Hangzhou, China). Her research has focused on the biocatalysis and biotransformation.

Xinxin Chen is studying as a Master candidate in College of Materials, Chemistry and Chemical Engineering (Hangzhou Normal University, Hangzhou, China). Her research has focused on the biocatalysis and biotransformation.

Xiaolin Pei, male, born in 1982, associated professor. He is working in College of Materials, Chemistry and Chemical Engineering (Hangzhou Normal University, Hangzhou, China). His research interest is biotechnology and bioengineering, mainly engaged in the directed evolution of enzymes and its catalysis application in the synthesis of chiral drugs.

Pengfei Zhang, male, born in 1965, professor. He obtained his Ph.D. degree in 2003. Recently, his research has focused on the synthesis of chiral compounds, green chemistry and medicinal chemistry.

Additional information

Funding

This study was supported by the National Natural Science Foundation of China [grant number 21576062]; Natural Science Foundation of Zhejiang Province [grant number LY18B060009], [grant number LY15B060011]; National Innovation and Entrepreneurship Training Program for Undergraduate [grant number 201710346013], [grant number 201810346008]; Scientific Research Innovation Fund for Graduate Students of Hangzhou Normal University; Research Plan for Sprout Talents in University in Zhejiang Province [grant number 2017R423027], and the Program for Innovative Research Team in Chinese University [grant number IRT1231].