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Abstract
Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.
Disclosure of Potential Conflicts of Interest
No potential conflicts of interest were disclosed.
Acknowledgments
We are very grateful to all lab members. We thank Claudia Miccolo for precious technical help. We thank all the support facilities at IEO and IFOM, in particular Andrea di Fonzo for the mass spectrometric analysis, Marisa Aliprandi for help in antibody production, Amanda Oldani and Sara Barozzi for technical support with imaging techniques. Most importantly, we thank Prof. Andrea Musacchio for his crucial help. This work was supported by the Associazione Italiana per la Ricerca sul Cancro (IG5732 and IG12075 to S.C.) and the Italian Ministry of Health (to S.C.). C.V.S. was supported by a Fondazione Umberto Veronesi fellowship. St.Sa. was supported by fellowships from the Italian Foundation for Cancer Research. C.S. was supported by the Austrian Science fund (FWF P25807)
Supplemental Material
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