Detection of chloramphenicol in chicken, pork and fish with a molecularly imprinted polymer-based microtiter chemiluminescence method

ABSTRACT

In this study, 4-nitrotoluene (NT) was used as dummy template to synthesize a molecularly imprinted polymer that was highly specific for chloramphenicol. The polymer was coated in the wells of 96-well microplates as recognition reagent to develop a chemiluminescence method. The analyte solution and an enzyme-labelled hapten were added into the wells to perform competition, and the light signal was induced with a highly efficient luminol-H₂O₂-4-(imidazol-1-yl)phenol system. Then, the optimized method was used to determine chloramphenicol in meat (chicken, pork and fish), and the limit of detection (LOD) was 5.0 pg g⁻¹. Furthermore, the polymer-coated plate could be reused four times, and one test could be finished within 20 min. The recoveries from the standard fortified blank meat samples were in the range of 71.5–94.4%. Therefore, this method could be used as a useful tool for routine screening the residue of chloramphenicol in meat samples.

Graphical Abstract

Assay procedures of the molecular imprinted polymer based chemiluminescence method for determination of chloramphenicol

KEYWORDS:

• 4-nitrotoluene
• chloramphenicol
• molecularly imprinted polymer
• chemiluminescence
• meat

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study is financed by Hebei Key Research and Development Project [18226628D] and Hebei Layer/Broiler Industry Technology System [HBCT2018150210].