Performance of allergen testing in a survey of frozen meals and meals ready-to-eat (MREs)

ABSTRACT

A 7-plex immunoassay capable of detecting cashew, egg, hazelnut, milk, peanut, shrimp, and soy allergens was used to screen meals ready-to-eat (MREs) and frozen meals that contained meat or poultry. The same food matrices were also evaluated using single individual allergen immunoassays. Multiplex and single allergen test results were compared with the allergen declared on the food label, which was considered the standard. For both the frozen meals (n = 113) and MREs (n = 24) each analytical method failed to detect allergens that were declared on product labels, but only in frozen meals were allergens detected that were not declared on the label. Undeclared allergens were detected for egg in 1.8% (2/113) and for soy in 7.1% (8/113) of frozen meals. Labelled allergens were not detected in 0.9% (1/113) of milk, 4.4% (5/113) of egg, and 15% (17/113) of soy allergens in frozen meals. Assay performance for evaluating allergens in MREs was poor.

GRAPHICAL ABSTRACT

KEYWORDS:

• Allergen
• multiplex immunoassay
• ELISA
• undeclared allergen
• food allergy

Acknowledgments

The authors wish to thank Keith Sorensen and Isaac Courneya for their assistance with sample processing. Dr. David Smith’s helpful comments are greatly appreciated.

Disclosure statement

No potential conflict of interest was reported by the author(s).