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Articles

A facile extraction method followed by UPLC-MS/MS for the analysis of aflatoxins and ochratoxin A in raw coffee beans

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Pages 1551-1560 | Received 05 Feb 2021, Accepted 21 Apr 2021, Published online: 28 May 2021
 

ABSTRACT

A UPLC-MS/MS method was developed and validated for the determination of aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) and ochratoxin A (OTA) in raw coffee samples. Mycotoxins were extracted using a modified QuEChERS method with little sample preparation excluding clean-up and enrichment procedures. Linearity was demonstrated for the five mycotoxins in the range 0.125–20 µg/kg. This method shows negligible matrix effect for individual concentrations, thus allowing the use of an external standard procedure. Limits of quantification (LOQ) ranged from 0.45 to 1 μg/kg. Recoveries between 63% and 89% were achieved. The intra- and inter-day precisions were lower than 15%. The applicability of the method was demonstrated, taking into account fitness for purpose, with simplicity, reliability, low costs and environmental friendliness. The results show that 3 out of 4 samples were contaminated with OTA.

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Acknowledgments

The authors gratefully acknowledge Laboratoire Marocain de l’Agriculture (LABOMAG), Casablanca, Morocco” for technical support.

Disclosure statement

No potential conflict of interest was reported by the author(s).

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