Effects of theasaponin E1 on the regulationglucose uptake of C2C12 myoblasts PI3K/Akt/mTOR signaling pathway

ABSTRACT

Targeting disorders of glucose metabolism and the occurrence of hyperglycemia provides a potential therapeutic strategy for balancing energy to reduce insulin resistance such as type 2 diabetes. The present study investigated whether theasaponin E1 regulate energy metabolism in skeletal muscle cells. C2C12 mouse myoblasts were differentiated into myotubes . An MTT colorimetric assay verified cell viability. Theasaponin E1 (30, 45 or 60 mg/ ml), or metformin (2.5 mM), insulin (150 nM) for reference were used to treat the C2C12 myotubes. The expressions of energy metabolism were measured by western blotting in C2C12 myotubes. 2-NBDG kit to detect the glucose uptake capacity. Theasaponin E1 Treatment increases glucose uptake,GSK 3 β, as well as increases to the glycogen synthesis through the PI3K/AKT/mTOR signaling pathway, while reducing AMPK and ACC activities, and reducing the adipose synthesis pathway. These results suggest that theasaponin E1 may be increases glucose uptake by improving muscle energy metabolism.

KEYWORDS:

• Theasaponin E1
• glucose uptake
• C2C12 myoblast

Acknowledgments

Thanks to Yunnan Agricultural University, the Key Laboratory of Pu’er Tea Ministry of Education and the Yunnan Plateau Characteristic Agricultural Industry Research Institute for funding and experimental guidance. We express our gratitude to Dr. Xuanjun Wang, Dr. Chongye Fang, and Dr. Yewei Huang for their helpful suggestions.

Disclosure statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

Author contributions

Ming Zhang, Zhiyun Chen, Di Tian protein assay, qPCR, Western blot and data analysis. ShaningWang, Yujie Huo, Juan Lu helped with protein experiments and some data analysis. Zaiqiao Li, Ling Song helped with qPCR. Xu Ji, Xiao Ma, Mingying Gu and Jun Sheng designed the study. MZ, XJ, XM, wrote this manuscript. All authors read and approved the final manuscript.

Data availability statement

Data available on request. The data underlying this article will be shared on reasonable request to the corresponding author.

Additional information

Funding

This work was funded by YEFICRC project of Yunnan provincial key programs [2019ZG009], S&T project of Yunnan provincial finance [YCJ2020-323] and Yunnan Youth Top-notch Talent Support Program [YNWR-QNBJ2018-173]