Extraction optimization, antioxidants, and lipid-lowering activities of polygonatum cyrtonema Hua polysaccharides

ABSTRACT

Polygonatum cyrtonema Hua polysaccharides (PCP) have beneficial effects on lipid metabolism and oxidative stress. Here, PCP extraction was optimized using response surface methodology (RSM), and the antioxidant and lipid-lowering effects of its main component, PCP1, were evaluated in vitro. The maximum PCP yield was 11.05% at an extraction temperature was 72°C, liquid-to-solid ratio of 21, extraction time of 96 min, and two extraction rounds. PCP1 was purified by column chromatography and showed significant scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and 2, 2'-azino-bis 3-ethylbenzthiazo-line-6-sulfonic acid (ABTS) radicals at concentration of 0.2–1.0 mg/mL. At a concentration of 200 $\mathit{\mu }$g/mL, PCP1 significantly improved lipid disorder and damage induced by palmitic acid in HepG2 cells. These results show that PCP has potent antioxidant and lipid-lowering effects in vitro; moreover, the PCP extraction rate is significantly increased using RSM. Therefore, PCP is an effective antioxidant and lipid-lowering product with potential medicinal applications.

KEYWORDS:

• Polygonatum cyrtonema Hua
• polysaccharide
• extraction optimization
• antioxidant activity
• lipid-lowering effect

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was financially supported by the Anhui Provincial Natural Science Foundation under grant [1908085MH248], Natural Science Foundation of the Education Department of Anhui Province under grant [KJ2021ZD0101], Science and Technology Project of Wuhu under grant 2022cg20, and Academic Funding Project for Top-notch Talents in Disciplines (Majors) of the Universities in Anhui Province under grant [gxbjZD2022043].