ABSTRACT
The uric acid (UA) induced damage influences human health and deserves attention. The purpose of this study was to investigate the protective effects of Skipjack trypsin hydrolysate (STH) and its Maillard product (STH-M) and the segmentation of STH-M (STH-M-S) against UA-induced HK-2 cells. The CCK8 assay was used to detect cell viability and flow cytometry was performed to check cell apoptosis and cell cycle and the functional mechanism was analyzed via transcriptome. The results showed STH-M-S could significantly improve the cell viability and inhibit cell apoptosis. Meanwhile, STH-M-S could influence the membrane and protein binding and regulate metabolic-related and immune-related pathways to deal with UA stress. Functional genes contained NEBL, KCNJ16, ABCA12, CLDN2, FLRT3, PRODH, KLHL14, VEPH1, HPGD (down regulated) and FOXL1, SCG5 (up regulated). Collectively, our results suggested that STH-M-S can alleviate cell injury induced by UA. STH-M-S could be an alternative to be applied for the treatment of hyperuricemia.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Credit authorship contribution statement
Authors listed in the manuscript have made substantial contributions to the conception and design of the work. Yurou Chu analyzed the data, draft the manuscript, conceptualize and validate the study. Jipeng Sun and Liang Chen participated in the investigation and samples collection. Ru Song and Xiaoyu Zou participated in the sequencing analyses. Jiaxing Wang designed, conceived and conceptualize the study, and reviewed the manuscript. All authors had read the manuscript and approved the final version to be published.
Supplementary material
Supplemental data for this article can be accessed online at https://doi.org/10.1080/19476337.2024.2352580